Consumers will be able to buy products from eBay merchants online and pick up the items from select Argos UK stores.
At least 50 eBay merchants will take part in the Click and Collect trial which is rolling out to around 150 Argos stores in "primary locations".
It's the first time the online marketplace has teamed up with a UK store chain in this way.
Argos expects 'Click & Collect' - which accounts for a third of the retailer's business - to grow further in the UK. 40 per cent of UK shoppers used some form of Click & Collect service during Christmas 2012.
Devin Wenig, President of eBay Marketplaces said: "The distinction between offline and online shopping continues to blur.
"Traditional retail isn’t going away; it is transforming. Smart retailers are innovating, reimagining the store and what it means to shop. We’re proud to join Argos on this journey - their unique store network and operating model is fit for serving customers in a digital future."
Argos MD John Walden commented: "With the continuing growth of online shopping, customers increasingly expect faster, cheaper and more convenient fulfilment of their orders.
Few companies move as many products as effectively as Argos through a national network of local stores. Having pioneered Click & Collect in 2000, it now accounts for around a third of our business and continues to grow.
"We look forward to assessing the opportunity for Argos to provide fulfilment for eBay’s merchants, including the operational requirements, attractiveness to sellers and consumers, and opportunity for increased customer footfall."